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. 2003 May;23(9):3116–3125. doi: 10.1128/MCB.23.9.3116-3125.2003

FIG. 1.

FIG. 1.

The Pph21 phosphatase in the pph21-102 allele is defective in its interaction with Tap42. (A) Wild-type (W.T.; Y689; lanes 1 and 2) and mutant cells (Y690; lanes 3 and 4) were grown to early log phase at 23°C and then shifted to 37°C for 2 h. Cell extracts were made and precipitated with anti-Tap42 antibody cross-linked to protein A beads. The presence of Tap42 (top) and HA-Pph21 (middle) in the precipitates was detected by Western blot analysis with anti-Tap42 and anti-HA antibodies, respectively. The amount of HA-Pph21 in the total cell extracts (Ext) was also determined (bottom). IP, immunoprecipitation. (B) Cell extracts from the same cells were incubated with anti-Tpd3 antibody. The precipitates were then analyzed by Western blotting to detect the presence of Tpd3 and HA-Pph21 with anti-Tpd3 and anti-HA antibodies, respectively.