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Tap42 is required for the cell cycle-dependent polarized distribution of actin. Exponentially growing yeast cells were shifted from 23 to 37°C for 4 h. Before (A and B) and after (C) the shift the cells were fixed, stained with fluorescent phalloidin, and imaged in fluorescence or DIC mode. (A) Wild-type cells (Y661) at 23°C; (B) tap42-11 mutant cells (Y176) at 23°C; (C) tap42-11 mutant cells (Y176) at 37°C.
