FIG. 3.

The Tap42 protein in the tap42-11 mutant cells is defective for interaction with phosphatases. Exponentially growing wild-type (W.T.; Y162) and tap42 mutant (Y703) cells were shifted from 23 to 37°C for 2 h. Cell extracts were made from cells before (lanes 1 and 3) and after (lanes 2 and 4) the shift and incubated with anti-Tap42 antibody cross-linked to protein A beads. The presence of Tap42 (top), HA-Pph21(middle), and Sit4 (bottom) in the precipitates (A) and in the cell extracts (Ext; B) was detected by Western blot analysis with anti-Tap42, anti-HA, and anti-Sit4 antibodies, respectively. IP, immunoprecipitation.