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The cell cycle-dependent organization of the actin cytoskeleton is sensitive to rapamycin treatment. Wild-type yeast cells expressing a control vector (pRS314) (A) or pJY182 (TOR1S1972R-pRS314) (B) were grown to early log phase at 23°C and treated with 300 nM rapamycin for 90 min. Cells were then fixed, stained with fluorescent phalloidin, and imaged in fluorescence and DIC modes.
