TABLE 3.

Quantitative analysis of actin defects in the tap42 and pph21 mutant cells^a

^aExponentially growing yeast cells were shifted from 23 to 37°C for 4 h. Before and after the shift the cells were fixed and stained for actin. The distribution of actin in the cells was examined with a confocal microscope, and the percentages of cells displaying polarized actin distribution at three different stages, unbudded, small to midsize budded, and large budded, of the cell cycle were quantified. At each stage, ≥200 cells for each strain were counted for actin distribution. The small- and midsize-budded cells are defined as those with a cross section bud diameter two-thirds that of the mother cell or less. The large-budded cells are those with a bud diameter more than two-thirds that of the mother cell. A polarized actin distribution is defined by the presence of an actin cap in the unbudded cells, by concentrated actin patches presented in the bud of the small- and midsize-budded cells, and by concentrated actin patches present at the mother-bud neck of the large-budded cells.

^b+, present; −, absent.