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. 2003 May;23(9):3320–3328. doi: 10.1128/MCB.23.9.3320-3328.2003

FIG. 3.

FIG. 3.

MLH1 and PMS2 possess functional NES. (A) MLH1-NES (MNES-GrG), PMS2-NES (PNES-GrG), and control (GrG) reporter constructs. GrG is an abbreviation for glucocorticoid receptor (Gr)-GFP (G) fusion, M refers to MLH1, and P refers to PMS2. All constructs were cloned into the pUHD10S vector. (B to J) Intracellular localization of GFP in HtTA-expressing cells transfected with MNES-GrG (B, E, and H), PNES-GrG (C, F, and I), or control construct GrG (D, G, and J). Panels B, C, and D show transfectants not treated with dexamethasone (DEX); panels E, F, and G represent transfectants treated with 1 μM dexamethasone for 30 min; panels H, I, and J represent cells treated with dexamethasone, followed by removal for two hours, in the presence of 30 μg of cycloheximide/ml. Confocal images were representative of at least 85% of 500 transfected cells examined and were collected and processed by using the settings explained in Materials and Methods.