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. 2003 May;23(9):3320–3328. doi: 10.1128/MCB.23.9.3320-3328.2003

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FIG. 6. — Formation and nuclear import of transspecies MutLα. (A) Western blot analysis of immunoprecipitation of Flag-MLH1 and HA-PMS2 in whole-cell lysates of HtTA cells nontransfected (lane 1), transfected with HA-PMS2 alone (lane 2), or transfected with HA-PMS2 and Flag-MLH1 (lane 3). Immunoprecipitation (IP) was with anti-Flag or anti-HA antibodies, as indicated. Precipitated proteins were resolved on SDS-6% PAGE and blotted onto PVDF membranes (WB), followed by probing with anti-Flag, anti-HA, or anti-MLH1 antibodies as indicated. Bands were visualized with the ECL detection kit. (B) Intracellular localization of mouse MLH1 in HeLa cells transfected with Flag-MLH1. Mouse MLH1 protein was detected with anti-Flag antibody. Image represents more than 95% of 500 transfectants examined.