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. 2003 May;23(9):3274–3286. doi: 10.1128/MCB.23.9.3274-3286.2003

FIG. 7.

FIG. 7.

Nuclear localization of A. thaliana pBrp/EYFP fusion protein in a fus6 mutant. (A) Localization of the A. thaliana pBrp/EYFP fluorescence in wild-type (FUS6) or CSN mutant (fus6) seedlings. The mutant was selected based on its phenotype and transiently transformed with the A. thaliana PBRP/EYFP construct. Panels a and d show A. thaliana pBrp/EYFP fluorescence in either wild-type (a) or mutant (d) seedlings. Panels b and e show corresponding chlorophyll red autofluorescence. Panels c and f show the overlap of EYFP, chlorophyll, and bright-field confocal images. Bars, 10 μm. (B) Nuclear localization of the A. thaliana pBrp/EYFP fluorescence in the transformed fus6 mutant cells. The EYFP fluorescence was observed in the DAPI-stained nucleus.