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Nuclear localization of endogenous A. thaliana pBrp protein in nuclei of Arabidopsis cells treated with proteasome inhibitor. Arabidopsis cells were preincubated with either 100 μM MG132 (MG.) or 2% DMSO (DM.) for 3h, and their nuclei were purified on Percoll gradients and analyzed by Western blotting with anti-pBrp, anti-RPB1, anti-IE37, and anti-LSU antibodies. A plastid fraction from leaves was used as a control (lane C).
