FIG. 6.

Restriction specificity of NK T cells activated for IFN-γ expression by E. coli challenge. Shown are hepatic lymphocytes from two representative mice of a single experiment. The mice were treated with either vehicle (A) or DEM (B) and then challenged with E. coli bacteria. (Left) Surface phenotypes of the total lymphocyte populations, including two subsets of double-positive cells (subsets A and B); (center) binding of α-GalCer-CD1d tetramers by these two NK T-cell subsets; (right) intracellular IFN-γ staining of the various subsets. This experiment was performed four times with similar results.