FIG. 2.

Immunohistochemical staining of adherent and internalized bacteria in fibroblasts. (A and B) Fibroblasts incubated with either strain Newman (A) or strain Newman mAH12 (B) at 37°C for 2 h were stained for expression of clumping factor by immunohistochemistry. The stainings were quantified by in situ imaging, and the data are presented as image analysis value corresponding to (frequency of positive area/total cell area) × mean intensity of positive area. (C) Imaging data from three separate experiments. Wilcoxon's rank sign test for paired samples revealed a significant difference between Newman and AH12 (P < 0.02).