FIG. 1.

Growth of WT (AP1) and mutant (RJ1) mtsABC strains under different environmental conditions. Experiments were performed at least three times with duplicate samples, and representative growth curves are shown. (A) Growth in THY under aerobic conditions. (B) Comparison of WT and mutant growth in THY or metal-depleted THY (Cx-THY) in a 5% CO₂ atmosphere. (C) Growth in a 5% CO₂ atmosphere in Cx-THY with 50 μM supplements of the following metal salts: ferric citrate, FeSO₄, MnCl₂, CuCl₂, ZnCl₂. (D) Growth in a 5% CO₂ atmosphere in Cx-THY supplemented with increasing concentrations (0 to 50 μM) of MnCl₂.