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Nitric oxide production by IFN-γ-activated murine macrophages in the presence of recombinant nematode cystatins. Peritoneal macrophages from BALB/c mice were activated with IFN-γ for 24 h and incubated with various concentrations of rCysele1, rCysele2, rOv17, and rOv33. The nitric oxide production of macrophages was determined in the culture supernatant by measuring nitrite. Data shown are means ± standard deviation of triplicate determinations.
