FIG. 4.
Secretion of TGF-β1 after i.n. administration of rBCG or nonrecombinant BCG. BALB/c mice were immunized i.n. with 5 × 106 cells of either nonrecombinant BCG (black bar) or rBCG (white bar) or received PBS (left) and were boosted 60 days later in the same way. BALF from each group of mice were collected and analyzed by ELISA for the presence of TGF-β1. Each sample was analyzed in triplicate. ∗, significant difference. Statistical significance was estimated by using the Mann-Whitney test (P < 0.05).