TABLE 1.
Summary of fluorescent MBP-Int280 and MBP-Int280CS bead binding to mucosal and splenic lymphocytes
| Lymphocyte | % Bindinga
|
|
|---|---|---|
| Int280 | Int280CS | |
| Splenic | 44 ± 6 | 1 ± 0.1 |
| Mesenteric lymph node | 44 ± 5 | 1 ± 0.5 |
| Peyer's patch | 36 ± 3 | 1 ± 0.3 |
| LPL | 24 ± 7 | 1 ± 0.5 |
| IEL | 10 ± 2 | 1.3 ± 0.3 |
a
Bead-to-cell ratio of 2:1. Results are means ± 1 SE of three experiments. Binding to LPL and IEL was significantly lower than binding to Peyer's patch, mesenteric lymph node, and spleen cells (P < 0.05; Mann-Whitney U test). A greater percentage of spleen cells and IEL were positive when the ratio of beads to cells was raised to 5:1, but the same pattern was seen in different tissues.