FIG. 2.

Characteristics of antibodies raised in mice by oral immunization with 25 μg of EcMSP4/5 and 10 μg of CTB. (A) Antibody level. (B) Epitope specificity. (C) Sensitivity of reduction. (D) Isotype distribution. All sera were measured at a 1:10,000 dilution. In panel A, the dotted line shows the mean antibody level induced by intraperitoneal immunization with the corresponding protein emulsified in Freund's adjuvant; in panels B, C, and D, the results are represented as the averages for six individually tested serum samples per group, and the error bars indicate the standard errors of the means. In panel C, antibody reactivities to nonreduced MSP4/5C (NR) and reduced and alkylated MSP4/5C (RA) are compared by using a Wilcoxon matched-pair test.