(A) A multicopy plasmid YEpSem1U, but not the vector plasmid alone, suppresses the growth of sec15–1 at 38°C. Two independent sec15–1 transformants are shown. (B) SEM1 is highly conserved in evolution. Comparison of several Sem1p homologues translated from dbEST database sequences. The fully conserved amino acids are shown in shaded boxes. R. communis, Ricinus communis; A. thaliana, Arabidopsis thaliana; C. elegans, Caenorhabditis elegans; B. malayi, Brugia malayi. (C) The degree of similarity and identity of different Sem1p homologues. The GenBank accession numbers for the homologues are R. communis, T14813; A. thaliana, T41856; C. elegans, D76210; B. malayi, R88405; mouse, X853589; rat, H35331; and human, U41515.