Figure 2.

SEM1 is implicated as a negative regulator of the exocyst complex. Wt cells, exocyst mutants sec3–2, sec5–24, sec8–9, sec10–2, and sec15–1, and double mutants sem1-Δ2 sec3–2, sem1-Δ2 sec5–24, sem1-Δ2 sec8–9, sem1-Δ2 sec10–2, and sem1-Δ2 sec15–1 were grown on YPD plates at 24°C or 37°C. Deletion of SEM1 suppresses the temperature-sensitive phenotype of sec3–2, sec8–9, sec10–2, and sec15–1, in contrast to sec5–24.