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Sem1p cosediments with Sec8-myc at 20 S in linear sucrose gradients. Yeast-cell lysate (NY1427) was centrifuged in a linear 10–30% (wt/vol) sucrose gradient containing 0.1% Triton X-100 for 8 hr. Sedimentation of Sem1p and Sec8p was analyzed from fractions by using SDS/PAGE and Western blotting. The sedimentation markers are BSA (4.5 S), catalase (11.5 S), and thyroglobulin (19.3S). The amount of total protein is presented in arbitrary units.
