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. 1999 Feb 2;96(3):909–914. doi: 10.1073/pnas.96.3.909

Figure 5.

Figure 5

Deletion of SEM1 in diploid S288C cells triggers pseudohyphal differentiation. Homozygous SEM1 deletants (sem1-Δ1/sem1-Δ1) were grown in low-nitrogen SLAD medium (16). After overnight growth, SEM1 deletants form asterisk-shaped colonies (b). These colonies contain clearly elongated cells forming short filaments in contrast to wt diploids in which the cells are round and the colonies are smooth (a). wt Σ1278b diploids form pseudofilaments on SLAD (c). In Σ1278b deleted for SEM1 (sem1-Δ1/sem1-Δ1) (d), pseudohyphae formation is enhanced compared with the wt cells (c).