FIG. 5.
Effects of the Stx1 B subunit (A), the CD77 MAb (B), and the Stx2 E166D mutant (C) on DNA fragmentation in HeLa cells. Cells were incubated for 4 h and analyzed for DNA fragmentation as described in Materials and Methods. In all panels, lane 1 contains no Stx1 and lane 2 contains Stx1 at 10 ng/ml. (A) Lanes 3 to 5, Stx1B at 78.5 ng/ml (lane 3), 785 ng/ml (lane 4), or 3.96 μg/ml (lane 5). (B) Lanes 3 and 4, CD77 MAb at 0.5 or 5 μg/ml, respectively. (C) Lanes 3 to 5, Stx2 E166D at 40 ng/ml (lane 3), 400 ng/ml (lane 4), or 4 μg/ml (lane 5).