FIG. 1.

(A to C) Chemical detection of glycosphingolipids (A) and chromatogram assay detection of their binding to Helicobacter hepaticus (B) and Helicobacter bilis (C). Lanes (where Cer is ceramide and Fuc is fucose): 1, acid glycosphingolipid fraction of human granulocytes, 40 μg; 2, Galβ4Glcβ1Cer (lactosylceramide) of dog intestine, 2 μg; 3, Galα3Galβ4Glcβ1Cer (isoglobotriaosylceramide) of dog intestine, 2 μg; 4, Galβ3GalNAcβ4Galβ4Glcβ1Cer (gangliotetraosylceramide) of mouse feces, 2 μg; 5, Galβ3(Fucα4)GlcNAcβ3Galβ4Glcβ1Cer (Le^a-5 glycosphingolipid) of human meconium, 2 μg; 6, Fucα2Galβ3(Fucα4)GlcNAcβ3Galβ4Glcβ1Cer (Le^b-6 glycosphingolipid) of human meconium, 2 μg; 7, GalNAcβ3Galα4Galβ4Glcβ1Cer (globotetraosylceramide) of human erythrocytes, 2 μg; 8, lactotetraosylceramide (Galβ4GlcNAcβ3Galβ4Glcβ1Cer) of human meconium, 2 μg.