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. 2003 May;71(5):2744–2757. doi: 10.1128/IAI.71.5.2744-2757.2003

FIG. 6.

FIG. 6.

IIA recognition of amastigotes, but not of trypomastigotes, of T. cruzi by immune sera from mice immunized with a plasmid containing the clone 9 gene. Intracellular amastigotes (A and B) or mixtures of extracellular amastigotes and trypomastigotes from cultures (C and D) were analyzed by IIA. Parasites were incubated with a pool of sera derived from mice immunized with plasmid pclone9 and imaged under Nomarski differential interference contrast (A and C) or fluorescence (B and D) microscopy. Bars, 10 and 50 μm.