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. 2003 May;71(5):2744–2757. doi: 10.1128/IAI.71.5.2744-2757.2003

FIG. 7.

FIG. 7.

Trypomastigote-induced parasitemia and mortality in BALB/c mice immunized with plasmids pclone9 and pSPclone9. BALB/c mice were immunized with pclone9, pSPclone9, or pcDNA3. Ten days after the last immunization, mice were challenged intraperitoneally with bloodstream trypomastigotes. (A) Course of infection, estimated by the number of trypomastigotes per milliliter of blood. Results are the means ± SDs obtained from mice immunized with pclone9 (n = 12), pSPclone9 (n = 12), or pcDNA3 (n = 10). At the peak of infection (day 8), the numbers of parasites in mice immunized with the different plasmids were compared by one-way ANOVA and Tukey HSD tests. In mice immunized with plasmid pclone9 or pSPclone9, we observed a significant reduction in the peak number of parasites from the number in animals immunized with pcDNA3 plasmid (P < 0.0001 by one-way ANOVA). There was no statistical difference between the numbers of parasites observed in mice immunized with pclone9 versus pSPclone9 (P > 0.05 by Tukey HSD test). (B) Kaplan-Meier curves for survival of mice immunized with pclone9 (n = 12), pSPclone9 (n = 12), or pcDNA3 (n = 10). The survival times for mice immunized with pclone9 or pSPclone9 were significantly different from that of mice immunized with pcDNA3 (control) (P < 0.0001 by log-rank test).