TABLE 1.
Characteristics of the plasmids used in this study
| Plasmid designation | Vector | Coding region of clone 9 gene (bp) | No. of translated aa of clone 9 gene | Additional sequence in the N-terminal regiona | No. of aa in polypeptide |
|---|---|---|---|---|---|
| pHIS-25kDa | pHIS | 1474-2085 | 492-694 | MSYYHHHHHHDYDIPTTENLYFQGAMDPM | 231 |
| pHIS-65kDa | pHIS | 232-2085 | 78-694 | MSYYHHHHHHDYDIPTTENLYFQGAMDPM | 645 |
| pclone9 | pcDNA3 | 1-2085 | 1-694 | None | 694 |
| pSPclone9 | pcDNA3 | 1-2085 | 1-694 | MKTIIALSYIFCLVFADYKDDDDAMDPLVTAASVLEFCRYPSHWRPILE | 742 |
| pIgSPclone9 | pcDNA3 | 1-2085 | 1-694 | MGMQVQIQSLFLLLLWVPGSRGGT | 718 |
| pIgSP | pcDNA3 | None | None | MGMQVQIQSLFLLLLWVPGSRG | 22 |
| pcDNA3 | pcDNA3 | None | None | None | 0 |
a
The sequences shown in boldface type are the hexahistidine tag (HHHHHH), influenza virus hemagglutinin (SP MKTIIALSYIFCLVFA), and mouse Ig κ chain SP (MQVQIQSLFLLLLWVPGSRG).