TABLE 3.
NO secretion by spleen cells of DNA-immunized mice upon in vitro restimulation with the recombinant protein His-65kDa
| Plasmida | Nitrite concn (μM)b
|
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|---|---|---|---|---|---|---|
| Expt 1
|
Expt 2
|
Expt 3
|
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| Medium | His-65kDa | Medium | His-65kDa | Medium | His-65kDa | |
| pcDNA3 | 3.24 ± 1.37 | 3.37 ± 0.22c | 2.73 ± 1.27 | 3.11 ± 0.58c | 4.32 ± 1.72 | 4.96 ± 1.56c |
| pclone9 | 2.22 ± 2.21 | 8.89 ± 0.48d | 1.65 ± 0.88 | 14.54 ± 1.12 | 6.86 ± 3.72 | 27.11 ± 1.16 |
| pSPclone9 | 4.19 ± 1.82 | 29.02 ± 1.64 | 1.46 ± 1.15 | 8.25 ± 0.67 | NTe | NT |
| pIgSPclone9 | 4.44 ± 5.56 | 27.74 ± 1.05 | 1.84 ± 1.15 | 5.27 ± 0.11 | 2.54 ± 1.77 | 20.83 ± 2.66 |
a
BALB/c mice were immunized with the indicated plasmid.
b
Two to five weeks after the last immunization, spleen cells were collected and stimulated in vitro with the recombinant protein His-65kDa at a final concentration of 1 μg/ml or not stimulated (medium). Results represent the means obtained from triplicate cultures ± SDs.
c
Not significantly different from values in the supernatants of cells cultured with medium only (P > 0.05 by the Student t test).
d
Values in boldface type are significantly different from values in the supernatants of cells cultured with medium only (P < 0.01 by the Student t test).
e
NT, not tested.