Figure 1.

Sequences of the murine and human LDLB cDNAs and LDLB expression in wild-type and mutant CHO cells. (A) Predicted amino acid sequence of murine ldlBp and alignment with a human ldlBp sequence assembled from the EST database (see Materials and Methods). The predicted amino acid sequence is numbered assuming that the first potential initiator codon is utilized. The human and mouse genes were aligned utilizing clustx. Identical amino acids are shaded and boxed. The double-headed, horizontal arrow indicates a gap in the human ldlBp sequence for which no information was available in the EST database. (B) Northern blot analysis (see Materials and Methods) of the LDLB mRNA in wild-type and mutant CHO cells by using LDLB (Upper) or G3PDH (Bottom) cDNA probes.