FIG. 1.

Schematic representation of the AP-PST technique utilized for detection of adult S. mansoni cDNAs encoding ORFs with N-terminal signal peptides. A randomly primed, size-selected S. mansoni cDNA library (adult stage) was constructed in the PST vector (6) and electroporated into MC1061/P3 E. coli. Transformants were patched onto pool plates with grids (52 colonies/plate). DNA from each pool was transfected into COS-7 cells and incubated for 72 h at 37°C. Cells were then fixed and stained for surface alkaline phosphatase activity.