. 2003 May;71(5):2548–2554. doi: 10.1128/IAI.71.5.2548-2554.2003

TABLE 1.

S. mansoni cDNA clones isolated from the PST-SmA library by using the alkaline phosphatase peptide signal trap

Clone(s)	Match(es), % identity, and GenBank accession no.	Kozak sequence^e	Putative signal sequence^b	No. of internal transmembrane domains	SignalP-NN^c				Signal P-HMM^d
Clone(s)	Match(es), % identity, and GenBank accession no.	Kozak sequence^e	Putative signal sequence^b	No. of internal transmembrane domains	C	S	Y	s	Signal P-HMM^d
p43F4	Tetraspanin: human CD63, 39%, and Sm23, 35%	`ttaggg_`	`MFGACMKNVCLLTTYCILLSILMVAEIAAGIFA IV...`	2	Y	Y	Y	Y	SP
p64A3	Tetraspanin: bovine CD9, 35%; M81720	`ttcatt_`	`MKGCIQCLRVILVVFNFLVVLIGLSVLGFS VY...`	2	Y	Y	Y	Y	SP
p97D2	S. mansoni eggshell protein EGG2, 100%; M21607	`xxaagc_`	`MKQSLTLVFLVAIGYATA HT...`	None	Y	Y	Y	Y	SP
p25G7, p112E4	S. mansoni eggshell protein EGG3, 100%; J03982	`tgaaaa_`	`MKQSLTLVFLVAIGYATA YT...`	None	Y	Y	Y	Y	SP
p33F5	S. mansoni EST,^a 100%; AA233973	`cgcatc_`	`MKYFICVIITVIIGVALS YS...`	None	Y	Y	Y	Y	SP
p83H3	S. mansoni EST,^a 100%; BE505109	`tcaatc_`	`MNRFFWTVTQCTILLVIICNLNTMKA TS...`	None	Y	Y	Y	Y	SP
p45H5	S. mansoni EST, 100%; BG931230	`tcggag_`	`MAASHACLDLRALLSVVGLLLASA GR...`	None	Y	N	Y	Y	SP
p76E2	S. mansoni EST, 100%; AA559399	`gtgccc_`	`MRFPAGTYDELQIPQGSWSELHKQHNKLYNKFFIVSATIATALFAAA FY...`	None	N	Y	Y	N	SA
p43G5	Unknown	`gtgtat_`	`MRKMPRFLSIHSGFLHILLS FY...`	None	N	N	Y	Y	SA
p30C2	Unknown	`atactg_`	`MMMIILMVLLSVIRIIIVGLISLVVVKG KL...`	None	Y	Y	Y	Y	SP
p25D5, p43F7, p101E3	Antisense S. mansoni ORF-RF2, 100%; M14309	`tccata_`	`MIHNSTGASVTASIMAGFVSATFAAFATFATTLTLTATTAITLSIIVAFTTTFSKAVAT VT...`	2	Y	Y	Y	Y	SA
p17C5	Antisense S. mansoni GST, 100%; M98271	`atattc_`	`MFYIFSLTNQLFNTIVFLVCFTHHMMFL RH...`	None	N	N	Y	Y	NS
p90C8	S. mansoni actin I, 100%; M80334		`MTQIMFETFNVPAMYVAIQAVLSLYASGRTTG IV...`	None	Y	Y	N	N	NS
p28C7	Human protein kinase DYR2,^f 46%; Q92630		`MHKAVIFSFPMIIWLIDMKFS NP...`	N/A^g	Y	N	Y	Y	NS
p25C4	S. mansoni Sm23,^f 100%; M34453		`MLLFYCLDNCALA NN...`	N/A	N	N	Y	N	NS

Clone sequence extends 5′ of the matching EST.

The putative cleavage point of each signal peptide is denoted by a space followed by the first two N-terminal residues of the processed protein.

SignalP-NN is based on neural networks.

SignalP-HMM is based on hidden Markov models. SP, N-terminal signal peptide; SA, signal anchor; NS, nonsecretory.

The Kozak consensus sequence for eukaryotes is GCCRCC_ where R is A or G and _ is an ATG start codon.

Incorrect reading frame drove surface expression.

N/A, not applicable.