TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| E. coli | ||
| DH5α | φ80dlacZ ΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR (rK− mK+) phoA supE44 λ-thi-1 gyrA96 relA1 | Life Technologies |
| MC4100 (λpir) | araD139 Δ(argF-lac)U169 rpsL150 relA1 flbB5301 deoC1 ptsF25 rbsR(λ pir) | 30 |
| S17.1 (λpir) | F−thi pro hsdR recA ΩpRP4 (Amps Tets Kans Tmpr) (λpir) | 18 |
| B. avium | ||
| 197N | parental B. avium strain; Nalr | 45 |
| PAS213 | fimC insertion mutant of B. avium 197N; fimC::mini-Tn5; Kanr Nalr | This study |
| PAS334 | fhaB insertion mutant of B. avium 197N; fhaB::mini-Tn5; Kanr Nalr | This study |
| PAS355 | fhaC insertion mutant of B. avium 197N; fhaC::mini-Tn5; Kanr Nalr | This study |
| PAS356 | bvgS insertion mutant of B. avium 197N; bvgS::mini-Tn5; Kanr Nalr | This study |
| Plasmids | ||
| pUTKm2 | Ampr KanroriR6K oriTRP4 | 10 |
| pLAFR5 | Broad-host-range vector; Tetr | 23 |
| pLAFR5-4a | pLAFR5 containing a 7-kbp segment of B. avium 197N DNA containing the fimB, fimC, fimD, and fhaC genes introduced into the BamHI and HindIII sites; Tetr | This study |
| pLAFR5-5a | pLAFR5 containing a 5-kbp segment of B. avium 197N DNA containing the fimB, fimC, and fimD genes introduced into the BamHI and HindIII sites; Tetr | This study |