FIG. 5.

Expression and purification of the passenger domain. (a) Extracts of the host strain uninduced (lane 1) and induced by 1 mM isopropyl-β-d-thiogalactopyranoside (lane 2), run on a 7.5% polyacrylamide gel. (b) Western blot of the same gel probed with a 1:1,000 dilution of anti-His₆ antibody (Novagen), showing the existence of the His₆ tag. (c) A Coomassie blue gel of the first four fractions from the purification of rAaePD. (d) Corresponding Western blot with conditions as for panel b. (e) Western blot of a SUNY 465 extract, with gel and probe conditions as for panel b. Note that the band is about ∼130 kDa, as seen in Fig. 3.