FIG. 2.

Immunoblot of F2310 (lvgA⁺) with LvgA antibody after trypsinization of sonicated bacterial cells or of whole bacterial cells with subsequent washing and sonication. Equivalent amounts of bacteria were added to each lane, as confirmed by Coomassie blue staining of gels before blot transfer. The trypsin concentrations (conc.) used are shown. The data are representative of findings of three independent experiments.