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. 2003 May;71(5):2827–2838. doi: 10.1128/IAI.71.5.2827-2838.2003

TABLE 2.

Primers, vectors, cloning sites, and recombinant plasmids used in gene cloning

Gene Strain Primers Vector Cloning sites Plasmid
set1 COL 5′-CCAGTACATATGAGTACATTAGAGGTTAGATCA-3′, 5′-CGGATCCTAATATAAATCGACTTCAATTT-3′ pET21b NdeI, BamHI pset2289
set2 COL 5′-CAGGTCATATGAAACAAAATCAAAAGTCAGTA-3′, 5′-AGGATCCTACTTTAAGTTAACTTCAATATC-3′ pET21b NdeI, BamHI pset2293
set3 COL 5′-CTGGTCATATGAAAGTAGAACTTGATGAGACA-3′, 5′-CGGATCCTAATTCAAATTCACTTCAATAT-3′ pET21b NdeI, BamHI pset2294
set4 8325 5′-CGGTTCATATGAAAGGAAAGTATGAAAAAATG-3′, 5′-CGGATCCTATTTCAAATTCACTTCGATGT-3′ pET21b NdeI, BamHI pset8325-1
set5 8325 5′-GCAGTTCATATGAAAGAAAAGCAAGAGAGAGTA-3′, 5′-CGGATCCTACTTACTTTAAATTTGTTTCA-3′ pET21b NdeI, BamHI pset8325-2
set6 8325 5′-CAGTACATATGGCAGAATCAACTCAAGGTCAA-3′, 5′-CGGATCCTATTTATATTCTAGCTCAACAT-3′ pET21b NdeI, BamHI pset8325-3
set7 8325 5′-CTGTAACCATGGGTGAACATAAAGCAAAATAT-3′, 5′-AGGATCCTATCTAATGTTGGCTTCTATTTT-3′ pET15 NcoI, BamHI pset8325-4
set8 COL 5′-GCAGCACATATGACAACACCATCTTCCACTAAA-3′, 5′-CGGATCCTATTTCAAATTCACTTCGATGT-3′ pET21b NdeI, BamHI pset2295
set9 COL 5′-CGGTCCATATGGAAAAAATACAATCAACT-3′, 5′-TGGATCCTATTTTATATTCACTTCAATTG-3′ pET21b NdeI, BamHI pset2298
set10 COL 5′-CCAGTTCATATGGAAAAGAAACCTATTGTAATA-3′, 5′-TGGATCCTATGCTTTTATAACTTTGATTTG-3′ pET21b NdeI, BamHI pset2300
set11 COL 5′-CAGTACATATGAAAGCAGAAGTTAAACAACAA-3′, 5′-AGGATCCTATTTCATTTCTACTAGAATTT-3′ pET21b NdeI, BamHI pset2301
HHS Vulnerability Disclosure