TABLE 2.
Primers, vectors, cloning sites, and recombinant plasmids used in gene cloning
Gene | Strain | Primers | Vector | Cloning sites | Plasmid |
---|---|---|---|---|---|
set1 | COL | 5′-CCAGTACATATGAGTACATTAGAGGTTAGATCA-3′, 5′-CGGATCCTAATATAAATCGACTTCAATTT-3′ | pET21b | NdeI, BamHI | pset2289 |
set2 | COL | 5′-CAGGTCATATGAAACAAAATCAAAAGTCAGTA-3′, 5′-AGGATCCTACTTTAAGTTAACTTCAATATC-3′ | pET21b | NdeI, BamHI | pset2293 |
set3 | COL | 5′-CTGGTCATATGAAAGTAGAACTTGATGAGACA-3′, 5′-CGGATCCTAATTCAAATTCACTTCAATAT-3′ | pET21b | NdeI, BamHI | pset2294 |
set4 | 8325 | 5′-CGGTTCATATGAAAGGAAAGTATGAAAAAATG-3′, 5′-CGGATCCTATTTCAAATTCACTTCGATGT-3′ | pET21b | NdeI, BamHI | pset8325-1 |
set5 | 8325 | 5′-GCAGTTCATATGAAAGAAAAGCAAGAGAGAGTA-3′, 5′-CGGATCCTACTTACTTTAAATTTGTTTCA-3′ | pET21b | NdeI, BamHI | pset8325-2 |
set6 | 8325 | 5′-CAGTACATATGGCAGAATCAACTCAAGGTCAA-3′, 5′-CGGATCCTATTTATATTCTAGCTCAACAT-3′ | pET21b | NdeI, BamHI | pset8325-3 |
set7 | 8325 | 5′-CTGTAACCATGGGTGAACATAAAGCAAAATAT-3′, 5′-AGGATCCTATCTAATGTTGGCTTCTATTTT-3′ | pET15 | NcoI, BamHI | pset8325-4 |
set8 | COL | 5′-GCAGCACATATGACAACACCATCTTCCACTAAA-3′, 5′-CGGATCCTATTTCAAATTCACTTCGATGT-3′ | pET21b | NdeI, BamHI | pset2295 |
set9 | COL | 5′-CGGTCCATATGGAAAAAATACAATCAACT-3′, 5′-TGGATCCTATTTTATATTCACTTCAATTG-3′ | pET21b | NdeI, BamHI | pset2298 |
set10 | COL | 5′-CCAGTTCATATGGAAAAGAAACCTATTGTAATA-3′, 5′-TGGATCCTATGCTTTTATAACTTTGATTTG-3′ | pET21b | NdeI, BamHI | pset2300 |
set11 | COL | 5′-CAGTACATATGAAAGCAGAAGTTAAACAACAA-3′, 5′-AGGATCCTATTTCATTTCTACTAGAATTT-3′ | pET21b | NdeI, BamHI | pset2301 |