TABLE 2.

Primers, vectors, cloning sites, and recombinant plasmids used in gene cloning

Gene	Strain	Primers	Vector	Cloning sites	Plasmid
set1	COL	5′-CCAGTACATATGAGTACATTAGAGGTTAGATCA-3′, 5′-CGGATCCTAATATAAATCGACTTCAATTT-3′	pET21b	NdeI, BamHI	pset2289
set2	COL	5′-CAGGTCATATGAAACAAAATCAAAAGTCAGTA-3′, 5′-AGGATCCTACTTTAAGTTAACTTCAATATC-3′	pET21b	NdeI, BamHI	pset2293
set3	COL	5′-CTGGTCATATGAAAGTAGAACTTGATGAGACA-3′, 5′-CGGATCCTAATTCAAATTCACTTCAATAT-3′	pET21b	NdeI, BamHI	pset2294
set4	8325	5′-CGGTTCATATGAAAGGAAAGTATGAAAAAATG-3′, 5′-CGGATCCTATTTCAAATTCACTTCGATGT-3′	pET21b	NdeI, BamHI	pset8325-1
set5	8325	5′-GCAGTTCATATGAAAGAAAAGCAAGAGAGAGTA-3′, 5′-CGGATCCTACTTACTTTAAATTTGTTTCA-3′	pET21b	NdeI, BamHI	pset8325-2
set6	8325	5′-CAGTACATATGGCAGAATCAACTCAAGGTCAA-3′, 5′-CGGATCCTATTTATATTCTAGCTCAACAT-3′	pET21b	NdeI, BamHI	pset8325-3
set7	8325	5′-CTGTAACCATGGGTGAACATAAAGCAAAATAT-3′, 5′-AGGATCCTATCTAATGTTGGCTTCTATTTT-3′	pET15	NcoI, BamHI	pset8325-4
set8	COL	5′-GCAGCACATATGACAACACCATCTTCCACTAAA-3′, 5′-CGGATCCTATTTCAAATTCACTTCGATGT-3′	pET21b	NdeI, BamHI	pset2295
set9	COL	5′-CGGTCCATATGGAAAAAATACAATCAACT-3′, 5′-TGGATCCTATTTTATATTCACTTCAATTG-3′	pET21b	NdeI, BamHI	pset2298
set10	COL	5′-CCAGTTCATATGGAAAAGAAACCTATTGTAATA-3′, 5′-TGGATCCTATGCTTTTATAACTTTGATTTG-3′	pET21b	NdeI, BamHI	pset2300
set11	COL	5′-CAGTACATATGAAAGCAGAAGTTAAACAACAA-3′, 5′-AGGATCCTATTTCATTTCTACTAGAATTT-3′	pET21b	NdeI, BamHI	pset2301