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Rabbit splenocyte mitogenicity of rSEL compared to that of TSST-1. Splenocytes (2 × 105/well) in quadruplicate wells of a microtiter plate were stimulated for 3 days with the indicated amounts of rSEL or TSST-1, and then 1 μCi of [3H]thymidine was added to each well. Cells were then reincubated for another day, and DNA was harvested onto glass fiber filters. Counts per minute were determined by scintillation counting. Negative-control wells (neg control) contained splenocytes only.
