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Reduced levels of PARP cleavage in gene knockout mice infected with T. gondii. The indicated WT and gene knockout animals were infected with 103 strain RH parasites, and PARP cleavage was assessed in splenocyte lysates from noninfected (NI) and 6-day-postinfection (I) samples. Full-length, active PARP appears at 116 kDa, and the caspase-cleaved inactive fragment is detected at 89 kDa. This experiment was repeated twice with similar results.
