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. 2003 May;71(5):2892–2896. doi: 10.1128/IAI.71.5.2892-2896.2003

FIG. 2.

FIG. 2.

RT-PCR analysis of luxS expression in wild-type B. burgdorferi 297 (lanes 1 to 6) and a luxS mutant (BbAH309) (lanes 7 to 12) cultured at 37°C (pH 6.8). + and −, PCRs with or without RT, respectively. Primer locations are depicted in Fig. 1B, and the primer pairs used are indicated above the pairs of the corresponding gel lanes. Lanes 2 and 4 show the operonic organization of pfs-1, metK, and luxS in wild-type 297. Lane 6 shows luxS expression in wild-type 297, whereas no similar RT-PCR product is detectable in the luxS mutant (lane 12). The absence of product in lane 10 is due to disruption of luxS by ermC.