FIG. 1.

Kinetic antibody responses (IgG) to E. canis whole-cell lysates in 15 dogs experimentally infected s.c. or i.d. with E. canis. The dog number is shown above each corresponding lane. A positive control (+) was included on days 0, 7, and 14, and a negative control (N) was included on days 21, 28, 35, 42, and 56 postinoculation. The day 0 immunoblot is representative of results obtained for days 7 and 14. Day 56 was 2 weeks postinitiation of doxycycline treatment (PT). Dogs 32, 33, 34, and 35 received a 1:100 inoculum dose i.d., and dogs 37, 41, 45, and 48 received the same dose s.c. Dogs 43 and 44 received a 1:500 inoculum dose i.d., and dogs 46, 51, and 52 received the same dose s.c. Dog 59 received a 1:1,000 inoculum dose i.d., and dog 54 received the same dose s.c. Protein standards representing 15 to 250 kDa (Precision protein standards; Bio-Rad) are shown to the right of each blot.