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Detection of anti-E. canis IgG2 responses to E. canis whole-cell antigen in dogs experimentally infected with E. canis on days 0, 7, 14, 21, 28, 35, 42, and 56 by ELISA. Sera were diluted 1:100, and values were plotted as the OD620. Dogs were inoculated with a 1:100 (top), 1:500 (middle), or 1:1,000 (bottom) dilution of E. canis.
