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Expression of the 25.3-kDa DtxR protein by the S. aureus MA2004 heterodiploid. The DtxR protein produced from the mntR promoter in the heterodiploid (lane 2) and native (lane 1) DtxR is recognized by an anti-DtxR antibody by Western blot analysis. The parent S. aureus strain containing an empty plasmid does not make a detectable cross-reacting protein, such as MntR, PerR, Zur, or Fur, the endogenous Fe-dependent regulators in S. aureus (lane 3).
