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. Author manuscript; available in PMC: 2006 Aug 7.
Published in final edited form as: J Immunol. 2006 Jun 15;176(12):7726–7735. doi: 10.4049/jimmunol.176.12.7726

FIGURE 3.

FIGURE 3

Binding of IL-2 to a subset of CD70+CD8+ T cells and association of binding with proliferation. A, The frequency of CD8+ cells that express CD70 and are bound to IL-2 was analyzed in PBMCs obtained from five patients following in vitro incubation with either low-dose (300 IU/ml) or high-dose (3,000 IU/ml) IL-2 for 5 days. B, Cell cycle analysis was conducted on samples of PBMCs that were cultured in 3,000 IU/ml IL-2 for 12 days. Cells that either bound to fluorescence-labeled rIL-2 (IL-2-FITC+) or did not bind detectable levels of IL-2 (IL-2-FITC−) were gated and analyzed for the percentage of cells in S phase. The mean ± SD of the percentage of gated populations from five PBMCs was calculated, and the significance of this difference was determined using the two-tailed Mann-Whitney U test. C, A kinetic analysis of the frequency of cells that bound to IL-2FITC in comparison to the frequency of cells that expressed CD27 and CD70 was conducted for two PBMC samples (from patients number 6 and number 9) that were incubated with high-dose IL-2 for a total of 12 days.