TABLE 1.

ORF-specific primers used to amplify fragments for recombinant expression

ORF	Sense primer(s)a	Antisense primer(s)a	Amplicon details
			Size (bp)	Portion of ORF
Tp0155	5′-ggatccaaccattgacacctgcc	5′-gaattctgcagctgaattatagaac	428	5′ end
	5′-ggatccttaacacgccgtcttcttc	5′-gaattccggaagggtacgcatac	630	3′ end
Tp0316 (TprF)	5′-ggatcctatgcaggcgtactcactccg	5′-gaattctcagcaagcaccccctgttcc	1,062	Internal
Tp0326 (Tp92)	5′-caggcaaacgacaattgg	5′-caaattatttaccgtgaacg	2,448	Full length
Tp0453	5′-ggatcccgtggaaggcatcagtag	5′-gaattccgaacttccctttttggag	758	Full length
Tp0483	5′-ggatccacgcggcgctcaaaaccg	5′-gaattcgttatgaaagcgatagccg	590	3′ end
Tp0557	5′-ggatccatccgcacgtttttatccgcac	5′-gaattcgggggcagtgtagcgcagg	636	Full length
Tp0620 (TprI)	5′-ggatcccgactcaccctcgaacca	5′-gaattcggtgagcaggtgggtgtag	633	Internal
Tp0751	5′-ggatccgggacaccgccgcacac	5′-gaattccttgcggtgtgtgtgcgc	360	5′ end
Tp0856	5′-gcggcgaagactcgctc	5′-cttgcgtccgagcagg	1,086	Full length
Tp0952	5′-ggatcctttccaagaagtcttcgaagtg	5′-gaattcagcgcccccgttaaaggg	502	5′ end
	5′-ggatcctgttgggacactcgatggg	5′-gaattcattcgaaatgttttgctcacag	542	3′ end
S. aureus LBPb	5′-ggatcctgccaattattacagatg	5′-aagcttttatttatctaagttatagaa	1,304	Full length

^aRestriction sites incorporated into the primers are underlined.

^bLBP, laminin-binding protein.