Figure 2.

Interaction of FOG-2 with GATA family members in yeast and in mammalian cells. (A) HF7c yeast cells were cotransformed with plasmids encoding GAL4 DNA-binding domain (DBD) and activation domain (ACT) fusion proteins as indicated. Three independent GATA-FOG-2 double transformants were replica plated on Trp-Leu- and Trp-Leu-His- plates along with indicated negative and positive controls. (B) Coimmunoprecipitation of FOG-2 with HA-GATA-4 in transfected 293 cells. Cells were transfected with plasmids expressing indicated proteins and nuclear lysates were immunoprecipitated with α-HA or α-FOG-2 antibody. Western blot analysis was performed with α-FOG-2 antibody. The position of FOG-2 is indicated.