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Northern blot analysis of adult (a) and embryonic (b) tissues of the mouse using FOG-2 cDNA. CLONTECH multiple-tissue Northern (MTN) blot membranes (a, CL7762–1; b, CL7763–1) were hybridized with a P32-labeled 0.9-kb BamHI-XbaI fragment of pCS2+FOG-2 (Upper). Position of the FOG-2 mRNA band is indicated by an arrow. To ensure the presence of RNA in every lane, blots were rehybridized for a ubiquitously expressed gene (Lower).
