Figure 3.
Tadpoles overexpressing GFP-D3 are resistant to exogenous T3. One-week-old tadpoles were screened by using GFP fluorescence and treated with 10 nM T3 for 1 wk. The reshaping of their heads and the protrusion of the lower jaw are the most obvious TH-induced changes. (A) Normal fertilized animals, no T3. (B) Normal fertilized animals treated with T3. (C) pCS2+GFP-D3 transgenic animals, no T3. (D) pCS2+GFP-D3 transgenic animals treated with T3. The arrowhead points to an animal showing partial response as evidenced by slight protrusion of the lower jaw. (E) Nontransgenic siblings, no T3. (F) Nontransgenic siblings treated with T3. (Bar = 4 mm.)