Figure 4. Effect of starvation on PDHK protein expression in skeletal muscle of wild-type and PDHK4−/− mice.
(A) Representative Western blots of PDHK4 and PDHK2 protein in gastrocnemius muscles of fed and 48 h starved wild-type (PDHK4+/+) and PDHK4−/− mice. (B) Histograms constructed from data obtained by Western-blot analysis, showing relative amounts of PDHK4 protein expressed in gastrocnemius muscle of fed and 48 h starved wild-type (PDHK4+/+) mice. n=4 in each group. (C) Histograms constructed from data obtained by Western-blot analysis, showing relative amounts of PDHK2 protein expressed in gastrocnemius muscle of fed and starved wild-type (PDHK4+/+) and PDHK4−/− mice. n=4 in each group.