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. 2006 Jul 13;397(Pt 3):501–508. doi: 10.1042/BJ20060276

Table 3. Yield of soluble protein, specific activity and kinetic parameters for the hydrolysis of methyl parathion by OpdA expressed in the presence (+) or absence (−) of CoCl2.

Protein Activity (μM/min per μl of culture) Soluble protein (mg/l) Specific activity (units/mg) kcat (s−1) Km (M) kcat/Km (s−1·M−1)
OpdA+Co2+ 1.94±0.12 4.9 168±3 1180±80 3.6×10−4±0.6×10−4 3.3×106±0.8×106
OpdA−Co2+ 0.14±0.01 1.8 34±1 255±16 4.1×10−4±0.6×10−4 6.3×105±1.3×105