Table 3. Yield of soluble protein, specific activity and kinetic parameters for the hydrolysis of methyl parathion by OpdA expressed in the presence (+) or absence (−) of CoCl2.
Protein | Activity (μM/min per μl of culture) | Soluble protein (mg/l) | Specific activity (units/mg) | kcat (s−1) | Km (M) | kcat/Km (s−1·M−1) |
---|---|---|---|---|---|---|
OpdA+Co2+ | 1.94±0.12 | 4.9 | 168±3 | 1180±80 | 3.6×10−4±0.6×10−4 | 3.3×106±0.8×106 |
OpdA−Co2+ | 0.14±0.01 | 1.8 | 34±1 | 255±16 | 4.1×10−4±0.6×10−4 | 6.3×105±1.3×105 |