PFGE of total SpeI-digested DNA of P. aeruginosa strains BM4529 (lane 1), BM4350 (lane 2), BM4531 (lane 3), BM4532 (lane 4), BM4533 (lane 5), BM4492 (lane 6), BM4534 (lane 7), and PAO38 (lane 8); concatemers of bacteriophage lambda were used as a molecular size standard (lane 9). Left, analysis of DNA by agarose gel electrophoresis; right, the resulting fragments were transferred to a Nytran membrane and hybridized to an ant(4′)-IIb probe labeled with 32P in vitro.