Figure 3.

SQV-7 is similar to LPG2, a protein required for GDP-mannose transport. (A) Cosmid C52E12 and plasmid clones assayed for sqv-7 rescue activity. + indicates that two or more independently transformed lines contained rescued sqv-7 animals. Otherwise symbols are used as in Fig. 1A. (B) Alignment of the SQV-7 and Leishmania LPG2 protein sequences and protein sequences predicted from two human cDNAs. Human 1 is predicted from cDNA accession no. D87449, and human 2 from clone 132056 (see text). The human 2 cDNA is likely to be incomplete at its 5′ end, which is indicated by three dots at the human 2 N terminus. Identities and predicted changes in mutant alleles are indicated as in Fig. 1B. (C) Kyte-Doolittle hydropathy plots of SQV-7 and Leishmania LPG2. In each case, the amino acid sequence is plotted along the horizontal axis, and its corresponding hydropathy (54) is plotted along the vertical axis. Regions above the central horizontal line are of positive hydropathy and, if sufficient length, therefore may be contained within transmembrane domains. The two plots are drawn at the same scale.