A–D: Immunolocalization of AQP-1 (A, B) and AQP-9 (C, D) in the efferent ductules of wild type (A, C) and αERKO (B, D) mice. In (A), AQP-1 expression is uniformly distributed over microvilli (thick arrows) and cilia (arrowheads) of nonciliated and ciliated cells, respectively. Basolateral staining (thin arrows) is also evident over the epithelium. In (B), there is absence of reaction over microvilli and cilia of many, but not all nonciliated and ciliated cells (curved arrows), and basolateral staining is dramatically reduced. In (C), AQP-9 expression is uniformly distributed over the microvilli (thick arrows) of nonciliated cells, but no staining of ciliated cells (arrowheads). In (D), there is absence of reaction over the microvilli of some nonciliated cells (curved arrows). Note the lumen (Lu) of αERKO mice (B, D) is enlarged compared to wild type mice (A, C). IT interbular space. 420X.