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. 2002 Feb;40(2):480–489. doi: 10.1128/JCM.40.2.480-489.2002

FIG. 1.

FIG. 1.

Dot-ELISA results of MAb LD5, MAb LF9, and MAb LE1 against boiled sonicates of various Leptospira serovars. Rows 1 to 6 of block I were blotted with antigens of serovars pomona, bratislava, bataviae, canicola, pyrogenes, and icterohaemorrhagiae (positive control), respectively; row 7 was blotted with PBS (negative control). Rows 1 to 7 of block II were blotted with antigens of serovars cynopteri, andamana, bangkok, grippotyphosa, hebdomadis, javanica, and icterohaemorrhagiae (positive control), respectively; row 8 was blotted with PBS (negative control). Rows 1 to 7 of block III were blotted with antigens of serovars autumnalis, bullum, djasiman, saigon, wolffi, patoc, and icterohaemorrhagiae (positive control); row 8 was blotted with PBS (negative control). Strips A were reacted with spent culture medium of P3x-63-Ag8.653 myeloma cells; strips B to D were reacted with MAb LD5, MAb LF9, and MAb LE1, respectively.